Depending on the number and morphological features of sperm, when fertilizing eggs with sperm, two methods are used: typical test tube baby (IVF) or microinjection (ICSI). Microinjection / ICSI. Injecting sperms into the ovum is called the microinjection [intracytoplasmic sperm injection, ICSI].
Today, many IVF (test-tube baby) clinics have started to apply ICSI in their test-tube procedures due to higher rates of success. If or whenever there is an option of ICSI, IVF has not been considered.
Microinjection applications have surpassed classical in-vitro fertilization (IVF) methods.
Today, many IVF [test-tube baby] clinics have started to apply ICSI in their test-tube procedures due to higher rates of success. If or whenever there is an option of ICSI, IVF has not been considered.
The history concerning ICSI development was first originally used in cases when fertilization was at risk, sperm and eggs were brought together and that for all other cases traditional IVF would be used.
It is a common question that confuses many of those seeking infertility treatments. The fact is, there is no difference between a test tube baby and IVF procedures. In other words, the two mean the same thing.
In a typical IVF procedure, eggs collected and sperm - the amount of which is calculated according to the number of eggs - are made ready and put together in a specially prepared medium of fluid. Within this medium, sperm and eggs do the fertilization process. In about 18 - 20 hours, it is checked for whether fertilization has occurred or not, and if yes, to what extent it has occurred. However, in the microinjection (ICSI) method, in order to fertilize the egg, sperm is directly injected into the egg by use of a special pipette. Microinjection is especially used in cases where there are issues regarding the number and morphology of the sperm, when on previous treatments, a/ there couldn’t be sufficient numbers of fertilization b/ recurrent unsuccessful IVF treatments.
Pregnancy rates increase significantly in such cases. During microinjection, the male’s sperm is placed into the cytoplasm in the female’s oocyte (egg), it is known as “Intra-Cytoplasmic Sperm Injection” (ICSI). ICSI has been revolutionary in terms of male infertility.
ICSI [microinjection] can be preferred in certain cases, such as
- When there was no fertilization with previous IVF treatments,
- Low fertilization rates on previous IVF treatments,
- When sperm is collected via surgical methods such as TESA/TESE
- When sperm parameters are not good.
Microinjection applications have surpassed classical in-vitro fertilization (IVF) methods…
What is a test tube baby?
The same thing happens in IVF Centres. A test tube baby gets its roots from the fact that sperms and the eggs are combined and kept to form an embryo [fertilized] in a test tube. In simple terms, it is the process where a baby is conceived artificially in ultra-hygienic laboratory conditions.
IVF and test tube baby have the same process since they are utilizing the same procedures for conception.
The test tube baby procedure is designed to assist couples with
- Unexplained infertility
- Blocked Fallopian Tubes
- Failed infertility treatment cycles
- Any other infertility case
The test tube baby process is simply divided into five steps;
- Infertility drugs and stimulation
Due to the fact that you are unable to conceive naturally, fertility drugs are given to stimulate your hormones and ovaries to produce more quality eggs for the procedure. Gonadotrophins, Clomifene Citrate, and Metformin are the common drugs given to a woman during egg stimulation.
Transvaginal ultrasounds and blood tests are frequently carried out to observe the egg follicles in the ovaries and to monitor the hormones.
- Egg retrieval
When the ultrasound procedures show that the eggs are ready, they are removed through a minor surgery (laparoscopic surgery) and taken for incubation.
Likewise, the man will have to deliver fresh sperms on the day of fertilization by masturbation or donor sperms are used
- Fertilization and embryo selection
The sperms are washed and then joined with the eggs in a process known as insemination. They are cultured in the laboratory for 5 days until cell division occurs.
- Endometrial Receptivity Analysis (ERA)
The latest procedure (genetic test) allows the doctor to determine whether the endometrial lining will facilitate implantation. The procedure developed by Igenomix, is a major breakthrough that will increase the success rate of test tube baby. When the woman’s uterus is receptive then the embryo transfer is made and if not, it is postponed
- Embryo transfer
Under the guidance of an ultrasound, a catheter is inserted into the woman’s vagina and the embryo is transferred to the uterus. She will need two hours of rest, after which she is free to get back to her work